Defeating Michael Morbius:
When you are trying to open the boarded up windows, if you are far away from him he will do a long jump towards you. Then it takes him a few seconds to recover to attack. Before he jumps, do not stand in the yellow circles. Instead, stand next to the wall opposite the windows. He will now jump to there, recover, etc. While he is recovering, pull down the boards. When he jumps into the light, attack him. Keep doing this until his and his wife's life runs out to complete the level.
He will fly around, etc. When he lines up, use the Spider-Wall to damage him and he will land. Attack him and repeat this strategy.
Defeating The Lizard:
You must first defeat The Hunter. Use the Black suit and it will be easy. You will then catch up with the new and improved Lizard. Stand by a generator and wait until he is about to strike, then dodge. He will hit the generator and shock himself. This will also shock you. Repeat this until you must counter-attack him and ride his back. Use the Left Analog-stick to balance and he will run into one. Then, the last one shuts off and you must turn it back on. Go over to the switch, and if done quickly enough, press Y to turn it back on. Ride his back one more time to defeat him.
She has a shield, so do not worry about her. Stand next to a satellite and wait until your Spidey senses activate. Press LT and dodge, causing her to damage her crystal. Repeat this until she runs away.
In your final encounter with Shriek, she temporarily takes away your Black suit, and figures from your imagination attack you (your loved ones). You can kill them with one hit, but it is pointless. Wait until the Black suit recharges, then switch over, revealing your true enemies. Attack Shriek until she changes you back to the old Spider-Man. Repeat the previous steps and she will collapse.
Defeating Michael Morbius and Shriek:
Wait until Michael is about to rush you, then open the window. He will be temporarily weakened. Beat him up. Shriek will heal him, losing some of her health. Repeat this until he dies, and she flees the scene.
In your first encounter with Sandman, do not try to attack him, just stay close to him. Counter-attack him several times on different levels to finally battle him. Unfortunately, you must use the Black suit to damage him. Just hit him around until you are prompted to give him a bath. Keep doing this cycle until you defeat him.
Sandman will create a mini-tornado and you cannot hit him. Use your Spidey senses to dodge the flying bars and wait until he throws a tank at you. Press B when prompted and send it back at him, allowing you to beat him up. Do not get over-confident, as he will start up his tornado and do some damage. Repeat this until he dies.
The second battle with Sandman is very easy. Just dodge when your Spidey senses alert you and run to the yellow circles when they appear. Complete the prompts to throw TNT barrels into Sandman. Eventually Harry will kill him, ending Sandman.
Before this battle, go to the upgrade menu and make sure you have the melee attack volley. Then, fight. Depending on your health level, you may or may not want to use the power up. At the moment, you can easily jump over Venom, confuse him, and chip away at his health. Keep doing this until his black suit begins to "glow". Now when you jump over him he will run away. This is a good opportunity to use your new attack and chip at him, occasionally giving some hits. Keep hitting him until his "glow" gets bigger and eventually Harry will tell you to knock Venom into the air. He'll fall back to the ground same as before, easily confused. Repeat this cycle until his health is really low and you can finish him off.
Use the following trick for an easy victory the first and second time you face Venom. When you meet Venom for the first time at the construction site, you must beat him down in five minutes or else Mary Jane will die. A easy way to do this is to defeat Venom in version one. It should be very easy). Version two is slightly harder, but all you have to do is watch his five punch combo. The last punch is his super punch. After he is done with his combo, beat him up. Repeat this until Venom turns into version three. You cannot do much once he is in his full power. Watch carefully and you should see a flashing symbol. Follow what the game instructs you to do and you should win. This works on both fights.
In the final battle with Venom, it is just the two of you. Repeat the previously listed steps to defeat him. When his "glow" is very high, run over to the metal bars and follow the prompt. This will slowly remove the suit off him. Just keep fighting him until he dies.