By satisfying the corresponding requirements indicated below, you can unlock additional characters for some portions of the game.
- Crimson Homura (Hebijo) - Beat the Hebijo story and you can play as Crimson Homura by holding L or R while selecting her
- Daidoji (Hanzo) - Clear the Hanzo story and then complete the Daidoji Appears! mission
- Master Suzune/Rin (Hebijo) - Clear the Hebijo story and complete the Strongest Shinobi mission